Welcome to Alexandrov lab
Identification of Disease-Associated Human RNA Pathways
We are state of the art equipped lab in the Clemson Center for Human Genetics (https://scienceweb.clemson.edu/chg/). We use forward genetics to identify and study disease-associated human pathways of RNA biogenesis, regulation, and surveillance.
To allow forward genetic interrogation of RNA pathways, we develop ultra high-throughput methods that employ CRISPR-based mutagenesis of the human genome and FACS (fluorescence-activated cell sorting).
The RNA pathways we are focusing on play critical roles in (a) cancer and (b) genetic diseases that arise from mutations resulting in a premature termination codon (PTC).
Among cancer-associated human RNA pathways, we are mainly focusing on those regulating levels of the human nuclear long non-coding RNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1).
Nonsense-mediated degradation (NMD) pathway that degrades mRNA carrying a premature termination codons (PTC) is the second major pathway of our interest.